pLOT Manual Wiki

  pLOT is a molecular biology tool for mapping plasmids and manipulating sequences.  It was initially intended
only for my own personal use when I couldn't find software to do exactly what I wanted.  Since I had used
freeware plasmid editing software back in grad school, I decided to release this for free, noncommercial use in
the hopes that it will help some poor grad student someday.  It is mostly written in very old school Visual Basic
6 with some functions written in assembly language.  pLOT can be used to create new DNA sequences or edit
existing ones.  It reads Genbank-style formats as well as ab1 chromatograms and certain other plasmid/sequence
manipulation program formats.  Projects can be saved to standard Genbank format, a modified a Genbank format
with additional, program-specific entries or to binary pLOT files when binary data such as chromatogram data is
present. pLOT Genbank files use the *.plt extension for the text based file or .pl2 for pLOT binary files.
  pLOT provides a number of tools for sequence manipulation, annotation and editing and incorporates an
expandable library of known and commonly used sequences for auto-detection in unannotated sequences as well as
the ability.  to insert or deleted sequences.  It also features restriction analysis tools for single enzymes
and enzyme mixes, virtual gel electrophoresis and many more.  It is a work in progress with new features added
as time goes on.